Beilstein J. Nanotechnol.2017,8, 1396–1406, doi:10.3762/bjnano.8.141
transport through them.
Keywords: Caco-2; differentiationandpolarisation; epithelial cell barrier; microscopy imaging; particle interaction; uptake and localisation; Introduction
An overall conclusion from a multitude of nanoparticle-cell in vitro studies is that nanoparticle uptake into cells is
nanoparticles such as microporous silicon [22], silica [23], poly(lactic-co-glycolic acid) [33] and carboxylated and aminated polystyrene [34].
We hypothesize that the low translocation observed in Caco-2 barriers results from a low uptake into the cells, an uptake that depends on cellular differentiationand
polarisation. Additionally, though it is known that biomolecules adsorbed to nanoparticles affect their cell adherence [35], uptake [26][35] and translocation [34], we wish to clarify their role in the full process, from adherence to translocation, in both undifferentiated and mature Caco-2 barriers.
To this
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Figure 1:
50 nm and 150 nm SiO2-NP association with Caco-2 barriers. Caco-2 barriers cultured for 4 and 21 da...